MetaboList – January 2015

——————————
Cancer metabolism
——————————

Pyruvate Kinase Isoform Expression Alters Nucleotide Synthesis to Impact Cell Proliferation
Sophia Y. Lunt, Vinayak Muralidhar, Aaron M. Hosios, William J. Israelsen, Dan Y. Gui, Lauren Newhouse, Martin Ogrodzinski, Vivian Hecht, Kali Xu, Paula N. Marín Acevedo, Daniel P. Hollern, Gary Bellinger, Talya L. Dayton, Stefan Christen, Ilaria Elia, Anh T. Dinh, Gregory Stephanopoulos, Scott R. Manalis, Michael B. Yaffe, Eran R. Andrechek, Sarah-Maria Fendt, Matthew G. Vander Heiden
http://www.cell.com/molecular-cell/abstract/S1097-2765(14)00837-5

Acetyl-CoA Synthetase 2 Promotes Acetate Utilization and Maintains Cancer Cell Growth under Metabolic Stress
Zachary T. Schug, Barrie Peck, Dylan T. Jones, Qifeng Zhang, Shaun Grosskurth, Israt S. Alam, Louise M. Goodwin, Elizabeth Smethurst, Susan Mason, Karen Blyth, Lynn McGarry, Daniel James, Emma Shanks, Gabriela Kalna, Rebecca E. Saunders, Ming Jiang, Michael Howell, Francois Lassailly, May Zaw Thin, Bradley Spencer-Dene, Gordon Stamp, Niels J.F. van den Broek, Gillian Mackay, Vinay Bulusu, Jurre J. Kamphorst, Saverio Tardito, David Strachan, Adrian L. Harris, Eric O. Aboagye, Susan E. Critchlow, Michael J.O. Wakelam, Almut Schulze, Eyal Gottlieb
http://www.cell.com/cancer-cell/abstract/S1535-6108(14)00511-X

Mitochondrial Genome Acquisition Restores Respiratory Function and Tumorigenic Potential of Cancer Cells without Mitochondrial DNA
An S. Tan, James W. Baty, Lan-Feng Dong, Ayenachew Bezawork-Geleta, Berwini Endaya, Jacob Goodwin, Martina Bajzikova, Jaromira Kovarova, Martin Peterka, Bing Yan, Elham Alizadeh Pesdar, Margarita Sobol, Anatolyj Filimonenko, Shani Stuart, Magdalena Vondrusova, Katarina Kluckova, Karishma Sachaphibulkij, Jakub Rohlena, Pavel Hozak, Jaroslav Truksa, David Eccles, Larisa M. Haupt, Lyn R. Griffiths, Jiri Neuzil, Michael V. Berridge
http://www.cell.com/cell-metabolism/abstract/S1550-4131(14)00554-3

IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo
Avinashnarayan Venkatanarayan, Payal Raulji, William Norton, Deepavali Chakravarti, Cristian Coarfa, Xiaohua Su, Santosh K. Sandur, Marc S. Ramirez, Jaehuk Lee, Charles V. Kingsley, Eliot F. Sananikone, Kimal Rajapakshe, Katherine Naff, Jan Parker-Thornburg, James A. Bankson, Kenneth Y. Tsai, Preethi H. Gunaratne & Elsa R. Flores
http://www.nature.com/nature/journal/v517/n7536/full/nature13910.html

Diet-Induced Unresolved ER Stress Hinders KRAS-Driven Lung Tumorigenesis
Giorgio Ramadori, Georgia Konstantinidou, Niranjan Venkateswaran, Tommasina Biscotti, Lorraine Morlock, Mirco Galié, Noelle S. Williams, Michele Luchetti, Alfredo Santinelli, Pier Paolo Scaglioni, Roberto Coppari
http://www.cell.com/cell-metabolism/abstract/S1550-4131%2814%2900551-8

Extracellular Metabolic Energetics Can Promote Cancer Progression
Jia Min Loo, Alexis Scherl, Alexander Nguyen, Fung Ying Man, Ethan Weinberg, Zhaoshi Zeng, Leonard Saltz, Philip B. Paty, Sohail F. Tavazoie
http://www.cell.com/cell/abstract/S0092-8674(14)01588-8

The Inhibitor Protein (IF1) of the F1F0-ATPase Modulates Human Osteosarcoma Cell Bioenergetics
Simona Barbato, Gianluca Sgarbi, Giulia Gorini, Alessandra Baracca, and Giancarlo Solaini
http://www.jbc.org/content/early/2015/01/20/jbc.M114.631788.abstract

Fumarate induces redox-dependent senescence by modifying glutathione metabolism
Liang Zheng, Simone Cardaci, Livnat Jerby, Elaine D. MacKenzie, Marco Sciacovelli, T. Isaac Johnson, Edoardo Gaude, Ayala King, Joshua D. G. Leach, RuAngelie Edrada-Ebel, Ann Hedley, Nicholas A. Morrice, Gabriela Kalna, Karen Blyth, Eytan Ruppin, Christian Frezza and Eyal Gottlieb
http://www.nature.com/ncomms/2015/150123/ncomms7001/full/ncomms7001.html

Breast-cancer-secreted ​miR-122 reprograms ​glucose metabolism in premetastatic niche to promote metastasis
Miranda Y. Fong, Weiying Zhou, Liang Liu, Aileen Y. Alontaga, Manasa Chandra, Jonathan Ashby, Amy Chow, Sean Timothy Francis O’Connor, Shasha Li, Andrew R. Chin, George Somlo, Melanie Palomares, Zhuo Li, Jacob R. Tremblay, Akihiro Tsuyada, Guoqiang Sun, Michael A. Reid, Xiwei Wu, Piotr Swiderski, Xiubao Ren, Yanhong Shi, Mei Kong, Wenwan Zhong, Yuan Chen  & Shizhen Emily Wang
http://www.nature.com/ncb/journal/v17/n2/full/ncb3094.html

——————
Treatments
——————

Synthetic lethality of combined glutaminase and Hsp90 inhibition in mTORC1-driven tumor cells
Jing Li, Alfredo Csibi, Sun Yang, Gregory R. Hoffman, Chenggang Li, Erik Zhang, Jane J. Yu, and John Blenis
http://www.pnas.org/content/112/1/E21.abstract.html?etoc

Genetic Disruption of Lactate/H^+ Symporters (MCTs) and Their Subunit CD147/BASIGIN Sensitizes Glycolytic Tumor Cells to Phenformin
Ibtissam Marchiq, Renaud Le Floch, Danièle Roux, Marie-Pierre Simon, and  Jacques Pouyssegur
http://cancerres.aacrjournals.org/content/75/1/171.abstract?etoc

l-Arginine Depletion Blunts Antitumor T-cell Responses by Inducing Myeloid-Derived Suppressor Cells
Matthew Fletcher, Maria E. Ramirez, Rosa A. Sierra, Patrick Raber, Paul Thevenot, Amir A. Al-Khami, Dulfary Sanchez-Pino, Claudia Hernandez, Dorota D. Wyczechowska, Augusto C. Ochoa, and Paulo C. Rodriguez
http://cancerres.aacrjournals.org/content/75/2/275.abstract?etoc

Dichloroacetate affects proliferation but not survival of human colorectal cancer cells
L. M. Delaney , N. Ho , J. Morrison , N. R. Farias , D. D. Mosser & B. L. Coomber
http://link.springer.com/article/10.1007/s10495-014-1046-4

Mechanism by which a recently discovered allosteric inhibitor blocks glutamine metabolism in transformed cells
Clint A. Stalnecker, Scott M. Ulrich, Yunxing Li, Sekar Ramachandran, Mary Kate McBrayer, Ralph J. DeBerardinis, Richard A. Cerione, and Jon W. Erickson
http://www.pnas.org/content/112/2/394.abstract.html?etoc

Blocking autophagy enhanced cytotoxicity induced by recombinant human arginase in triple-negative breast cancer cells
Z Wang, X Shi, Y Li, J Fan, X Zeng, Z Xian, Z Wang, Y Sun, S Wang, P Song, S Zhao, H Hu and D Ju
http://www.nature.com/cddis/journal/v5/n12/abs/cddis2014503a.html?WT.ec_id=CDDIS-201412

Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate
H Nilsson, D Lindgren, A Mandahl Forsberg, H Mulder, H Axelson and M E Johansson
http://www.nature.com/cddis/journal/v6/n1/abs/cddis2014545a.html

————
mTOR
————

Lysosomal amino acid transporter SLC38A9 signals arginine sufficiency to mTORC1
Shuyu Wang, Zhi-Yang Tsun, Rachel L. Wolfson, Kuang Shen, Gregory A. Wyant, Molly E. Plovanich, Elizabeth D. Yuan, Tony D. Jones, Lynne Chantranupong, William Comb, Tim Wang, Liron Bar-Peled, Roberto Zoncu, Christoph Straub, Choah Kim, Jiwon Park, Bernardo L. Sabatini, and David M. Sabatini
http://www.sciencemag.org/content/347/6218/188.full?utm_source=eloqua

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1
Manuele Rebsamen, Lorena Pochini, Taras Stasyk, Mariana E. G. de Araújo, Michele Galluccio, Richard K. Kandasamy, Berend Snijder, Astrid Fauster, Elena L. Rudashevskaya, Manuela Bruckner, Stefania Scorzoni, Przemyslaw A. Filipek, Kilian V. M. Huber, Johannes W. Bigenzahn, Leonhard X. Heinz, Claudine Kraft, Keiryn L. Bennett, Cesare Indiveri, Lukas A. Huber & Giulio Superti-Furga
http://www.nature.com/nature/journal/vaop/ncurrent/full/nature14107.html

Differential regulation of mTORC1 by leucine and glutamine
Jenna L. Jewell, Young Chul Kim, Ryan C. Russell, Fa-Xing Yu, Hyun Woo Park, Steven W. Plouffe, Vincent S. Tagliabracci, Kun-Liang Guan
http://www.sciencemag.org/content/347/6218/194.full?utm_source=eloqua

Ligand-Occupied Integrin Internalization Links Nutrient Signaling to Invasive Migration
Elena Rainero, Jonathan D. Howe, Patrick T. Caswell, Nigel B. Jamieson, Kurt Anderson, David R. Critchley, Laura Machesky, Jim C. Norman
http://www.cell.com/cell-reports/abstract/S2211-1247(14)01066-3

mTORC1 Phosphorylates UVRAG to Negatively Regulate Autophagosome and Endosome Maturation
Young-Mi Kim, Chang Hwa Jung, Minchul Seo, Eun Kyoung Kim, Ji-Man Park, Sun Sik Bae, Do-Hyung Kim
http://www.cell.com/molecular-cell/abstract/S1097-2765(14)00908-3

TORC1 Inhibition Induces Lipid Droplet Replenishment in Yeast
Juliana B. Madeira, Claudio A. Masuda, Clarissa M. Maya-Monteiro, Gabriel Soares Matos, Mónica Montero-Lomelí, and Bruno L. Bozaquel-Morais
http://mcb.asm.org/content/35/4/737.abstract?etoc

p53 suppresses carcinoma progression by inhibiting mTOR pathway activation
N Akeno, A L Miller, X Ma and K A Wikenheiser-Brokamp
http://www.nature.com/onc/journal/v34/n5/abs/onc2013589a.html

—————————–
Starvation, AMPK
—————————–

Autophagy mediates nonselective RNA degradation in starving yeast
Evelyn Welter and Zvulun Elazar
http://EMBOJ.embopress.org/content/34/2/131?etoc

The Energy Sensor AMPK Regulates T Cell Metabolic Adaptation and Effector Responses In Vivo
Julianna Blagih, François Coulombe, Emma E. Vincent, Fanny Dupuy, Gabriela Galicia-Vázquez, Ekaterina Yurchenko, Thomas C. Raissi, Gerritje J.W. van der Windt, Benoit Viollet, Erika L. Pearce, Jerry Pelletier, Ciriaco A. Piccirillo, Connie M. Krawczyk, Maziar Divangahi, Russell G. Jones
http://www.cell.com/immunity/abstract/S1074-7613(14)00497-X

Polyamine Metabolism is Sensitive to Glycolysis Inhibition in Human Neuroblastoma Cells
M. Victoria Ruiz-Pérez, Miguel Ángel Medina, José Luis Urdiales, Tuomo A. Keinänen, and Francisca Sánchez-Jiménez
http://www.jbc.org/content/early/2015/01/15/jbc.M114.619197.abstract

ATP-citrate lyase regulates cellular senescence via an AMPK- and p53-dependent pathway
Jong-Hyuk Lee, Hyonchol Jang, Soon-Min Lee, Ji-Eun Lee, Jinmi Choi, Tae Wan Kim, Eun-Jung Cho and Hong-Duk Youn
http://onlinelibrary.wiley.com/doi/10.1111/febs.13139/abstract?campaign=woletoc

—————————–
Hypoxia and HIFs
—————————–

HIF1α and HIF2α exert distinct nutrient preferences in renal cells.
Arreola A, Cowey CL, Coloff JL, Rathmell JC, Rathmell WK.
http://www.ncbi.nlm.nih.gov/pubmed/24879016

Hypoxia-specific ultrasensitive detection of tumours and cancer cells in vivo
Xianchuang Zheng, Xin Wang, Hui Mao, Wei Wu, Baorui Liu and Xiqun Jiang
http://www.nature.com/ncomms/2015/150105/ncomms6834/full/ncomms6834.html

TAp73 suppresses tumor angiogenesis through repression of proangiogenic cytokines and HIF-1α activity
Marina Stantic, Habib A. M. Sakil, Hanna Zirath, Trixy Fang, Gema Sanz, Alejandro Fernandez-Woodbridge, Ana Marin, Evelyn Susanto, Tak W. Mak, Marie Arsenian Henriksson, and Margareta T. Wilhelm
http://www.pnas.org/content/112/1/220.abstract.html?etoc

TAp73 opposes tumor angiogenesis by promoting hypoxia-inducible factor 1α degradation
Ivano Amelio, Satoshi Inoue, Elke K. Markert, Arnold J. Levine, Richard A. Knight, Tak W. Mak, and Gerry Melino
http://www.pnas.org/content/112/1/226.abstract.html?etoc

UCHL1 provides diagnostic and antimetastatic strategies due to its deubiquitinating effect on HIF-1a
Yoko Goto, Lihua Zeng, Chan Joo Yeom, Yuxi Zhu, Akiyo Morinibu, Kazumi Shinomiya, Minoru Kobayashi, Kiichi Hirota, Satoshi Itasaka, Michio Yoshimura, Keiji Tanimoto, Masae Torii, Terumasa Sowa, Toshi Menju, Makoto Sonobe, Hideaki Kakeya, Masakazu Toi, Hiroshi Date, Ester M. Hammond, Masahiro Hiraoka & Hiroshi Harada
http://www.nature.com/ncomms/2015/150123/ncomms7153/full/ncomms7153.html

———————–
Miscellaneous
———————–

VEGF-Targeted Therapy Stably Modulates the Glycolytic Phenotype of Tumor Cells
Matteo Curtarello, Elisabetta Zulato, Giorgia Nardo, Silvia Valtorta, Giulia Guzzo, Elisabetta Rossi, Giovanni Esposito, Aichi Msaki, Anna Pastò, Andrea Rasola, Luca Persano, Francesco Ciccarese, Roberta Bertorelle, Sergio Todde, Mario Plebani, Henrike Schroer, Stefan Walenta, Wolfgang Mueller-Klieser, Alberto Amadori, Rosa Maria Moresco, and Stefano Indraccolo
http://cancerres.aacrjournals.org/content/75/1/120.abstract?etoc

PFKFB4 controls embryonic patterning via Akt signalling independently of glycolysis
Caterina Pegoraro, Ana Leonor Figueiredo, Frédérique Maczkowiak, Celio Pouponnot, Alain Eychène and Anne H. Monsoro-Burq
http://www.nature.com/ncomms/2015/150120/ncomms6953/full/ncomms6953.html

Genetic dissection of leukemia-associated IDH1 and IDH2 mutants and D-2-hydroxyglutarate in Drosophila
Zachary J. Reitman, Sergey A. Sinenko, Eric P. Spana, and Hai Yan
http://www.bloodjournal.org/content/125/2/336.long

Pyruvate Kinase M2 Regulates Hif-1α Activity and IL-1β Induction and Is a Critical Determinant of the Warburg Effect in LPS-Activated Macrophages
Eva M. Palsson-McDermott, Anne M. Curtis, Gautam Goel, Mario A.R. Lauterbach, Frederick J. Sheedy, Laura E. Gleeson, Mirjam W.M. van den Bosch, Susan R. Quinn, Raquel Domingo-Fernandez, Daniel G.W. Johnson, Jain-kang Jiang, William J. Israelsen, Joseph Keane, Craig Thomas, Clary Clish, Matthew Vanden Heiden, Ramnik J. Xavier, Luke A.J. O’Neill
http://www.cell.com/cell-metabolism/abstract/S1550-4131(14)00556-7

Loss of autophagy causes a synthetic lethal deficiency in DNA repair
Emma Y. Liu, Naihan Xu, Jim O’Prey, Laurence Y. Lao, Sanket Joshi, Jaclyn S. Long, Margaret O’Prey, Daniel R. Croft, Florian Beaumatin, Alice D. Baudot, Michaela Mrschtik, Mathias Rosenfeldt, Yaou Zhang, David A. Gillespie, and Kevin M. Ryan
http://www.pnas.org/content/112/3/773.abstract.html?etoc

Metabolic programming and PDHK1 control CD4+ T cell subsets and inflammation
Valerie A. Gerriets, Rigel J. Kishton, Amanda G. Nichols, Andrew N. Macintyre, Makoto Inoue, Olga Ilkayeva, Peter S. Winter, Xiaojing Liu, Bhavana Priyadharshini, Marta E. Slawinska, Lea Haeberli, Catherine Huck, Laurence A. Turka, Kris C. Wood, Laura P. Hale, Paul A. Smith, Martin A. Schneider, Nancie J. MacIver, Jason W. Locasale, Christopher B. Newgard, Mari L. Shinohara, Jeffrey C. Rathmell
http://www.jci.org/articles/view/76012

Rapid Analysis of Glycolytic and Oxidative Substrate Flux of Cancer Cells in a Microplate
Lisa S. Pike Winer, Min Wu
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109916

—————-
Reviews
—————-

Nutrient-sensing mechanisms and pathways
Alejo Efeyan, William C. Comb, David M. Sabatini
http://www.nature.com/nature/journal/v517/n7534/full/nature14190.html

The role for autophagy in cancer
Eileen White
http://www.jci.org/articles/view/73941

Autophagy in cellular metabolism and cancer
Xuejun Jiang, Michael Overholtzer, Craig B. Thompson
http://www.jci.org/articles/view/73942

Lon protease: A key enzyme controlling mitochondrial bioenergetics in cancer
Pedro M Quirós, Clea Bárcena & Carlos López-Otín
http://www.tandfonline.com/doi/abs/10.4161/23723548.2014.968505

Targeting tumor cell metabolism via the mevalonate pathway: Two hits are better than one
Aleksandra Pandyra & Linda Z Penn
http://www.tandfonline.com/doi/abs/10.4161/23723548.2014.969133

Cellular and metabolic functions for autophagy in cancer cells
Candia M. Kenific, Jayanta Debnath
http://www.cell.com/trends/cell-biology/abstract/S0962-8924(14)00158-5

TAK1-dependent autophagy: A suppressor of fatty liver disease and hepatic oncogenesis
Ekihiro Seki
http://www.tandfonline.com/doi/abs/10.4161/23723548.2014.968507?ai=1gumh&ui=79l0u&af=T

Hexokinase II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy
D J Roberts and S Miyamoto
http://www.nature.com/cdd/journal/v22/n2/abs/cdd2014173a.html?WT.ec_id=CDD-201502

mTOR: a pharmacologic target for autophagy regulation
Young Chul Kim, Kun-Liang Guan
http://www.jci.org/articles/view/73939

Pluripotent stem cell energy metabolism: an update
Tara Teslaa and Michael A Teitell
http://EMBOJ.embopress.org/content/34/2/138?etoc

The role of hypoxia-inducible factor-2 in digestive system cancers
J Zhao, F Du, G Shen, F Zheng and B Xu
http://www.nature.com/cddis/journal/v6/n1/abs/cddis2014565a.html

Current treatment strategies for inhibiting mTOR in cancer
Francesca Chiarini, Camilla Evangelisti, James A. McCubrey, Alberto M. Martelli
http://www.cell.com/trends/pharmacological-sciences/abstract/S0165-6147(14)00195-3

Redox control of glutamine utilization in cancer
L Alberghina and D Gaglio
http://www.nature.com/cddis/journal/v5/n12/abs/cddis2014513a.html?WT.ec_id=CDDIS-201412

Cellular metabolism in colorectal carcinogenesis: Influence of lifestyle, gut microbiome and metabolic pathways
Hanne R. Hagland, Kjetil Søreide
http://www.cancerletters.info/article/S0304-3835(14)00133-5/abstract


Biochemical Pharmacology
Special issue Edited by Marc Diederich
“Alterations of Metabolic Pathways as Therapeutic Targets”
http://www.sciencedirect.com/science/journal/00062952/92/1

Including,

– Glycogen metabolism in cancer
Christos E. Zois, Elena Favaro, Adrian L. Harris
– Heterogeneity of glycolysis in cancers and therapeutic opportunities
Marc O. Warmoes, Jason W. Locasale
– Modulatory roles of glycolytic enzymes in cell death
Claudia Cerella, Mario Dicato, Marc Diederich
– Metabolic orchestration between cancer cells and tumor microenvironment as a co-evolutionary source of chemoresistance in ovarian cancer: A therapeutic implication
Dong Hoon Suh, Hee Seung Kim, Boyun Kim, Yong Sang Song
– Mitochondrial dysfunction in cancer chemoresistance
Nicoletta Guaragnella, Sergio Giannattasio, Loredana Moro
– Targeting antioxidants for cancer therapy
Andrea Glasauer, Navdeep S. Chandel
– Energy restriction mimetic agents to target cancer cells: Comparison between 2-deoxyglucose and thiazolidinediones
Sandra Kuntz, Sabine Mazerbourg, Michel Boisbrun, Claudia Cerella, Marc Diederich, Isabelle Grillier-Vuissoz, Stephane Flament
– PARP and other prospective targets for poisoning cancer cell metabolism
Judith Michels, Florine Obrist, Maria Castedo, Ilio Vitale, Guido Kroemer

—————–
Comments
—————–

Making sense of amino acid sensing
Robert T. Abraham
http://www.sciencemag.org/content/347/6218/128.full?utm_source=eloqua

The Metabolic Milieu of Metastases
William J. Sullivan, Heather R. Christofk
http://www.cell.com/cell/abstract/S0092-8674(15)00066-5

AMPK Helps T Cells Survive Nutrient Starvation
Kai Yang, Hongbo Chi
http://www.cell.com/immunity/abstract/S1074-7613(14)00496-8

Essential Role for Oxidative Phosphorylation in Cancer Progression
Maria Chiara Maiuri, Guido Kroemer
http://www.cell.com/cell-metabolism/abstract/S1550-4131(14)00564-6

ZMP: A Master Regulator of One-Carbon Metabolism
Gregory S. Ducker, Joshua D. Rabinowitz
http://www.cell.com/molecular-cell/abstract/S1097-2765(15)00013-1

Acetate nourishes stressed tumour cells
http://www.nature.com/nrc/journal/v15/n2/full/nrc3899.html

The mitochondria thief
http://www.nature.com/nrc/journal/v15/n2/full/nrc3901.html

IDH1 Inhibitor Shows Promising Early Results
http://cancerdiscovery.aacrjournals.org/content/5/1/4.2.abstract?etoc

Induction of Metabolic Reprogramming Suppresses p53-Null Tumor Growth
http://cancerdiscovery.aacrjournals.org/content/5/1/11.2.abstract?etoc

Metabolism: Feed a cold, starve a tumour
http://www.nature.com/nrc/journal/v15/n1/full/nrc3881.html?WT.ec_id=NRC-201501

Hypoxia and Hippo Signaling
http://cancerdiscovery.aacrjournals.org/content/5/1/11.2.abstract?etoc

Getting Specific About Amino Acid Sensing
http://stke.sciencemag.org/content/8/359/ec11.abstract?utm_source=eloqua

Sensing Amino Acids at the Lysosome
http://stke.sciencemag.org/content/8/359/ec12.abstract?utm_source=eloqua

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

%d bloggers like this: